Electron Microanalysis Core Facility
EDS simulation (CASINO)
List of bacteria for which new antibiotics are urgently needed (link)
CDC (link)
READING
Chemical biology of peptidoglycan acetylation and deacetylation (link, pdf)
Assay for PG O-acetyl transferase Assay
O-Acetylated PG controlling the activity of bacterial autlysins (link pdf)
Structural variation in the glycan strands of bacterial peptidoglycan (link, pdf)
PDZ domain (link)
FEMS Microbiology Reviews (special issue, link)
POSTDOC
Eight Attributes of Highly Sucessful Postdocs (Science 1999)
A day in the life of a topflight lab (Science 1999)
Staphylococcus aureus Standard Media (Protocol)
| Salts | 1L | 10X | |
| KH2PO4 | 1 g | 10 g | Use KCl and NaOH to adjust the pH to 7.0. Use 100mL of 10X Salt Stock for 1L of SASM |
| K2HPO4·3H2O | 1 g | 10 g | |
| (NH4)2SO4 | 1 g | 10 g | |
| NaCl | 10 mg | 100 mg | |
| Carbon Source | 1L | ||
| D-Glucose | 10 g | 20% stock solution can be prepared | |
| Vitamines | 1L | 125X (500ml) | |
| Pyridoxine·HCl | 1 mg | 62.5 mg | Light shield the stock solution and keep it at 4 C.Use 8ml of 125X vitamin stock solution to final 1L of SASM. |
| Calcium Pantothenate (B5) | 2 mg | 125 mg | |
| Niacin (nicotinic acid) | 2 mg | 125 mg | |
| Riboflavin | 1 mg | 62.5 mg | |
| Folid acid | 0.1 mg | 6.25 mg | |
| Thiamine·HCl | 2 mg | 125 mg | |
| Biotin (B7) | 0.1 mg | 6.25 mg | |
| Inositol | 1 mg | 62.5 mg | |
| Nucleosides | 1L | ||
| Adenine | 5 mg | Add directly. Stock solution cannot be prepared due to low solubility of nucleosides. Heating (autoclave) helps. | |
| Uracil | 5 mg | ||
| Cytosine | 5 mg | ||
| Guanine | 5 mg | ||
| Xanthine | 5 mg | ||
| Trace Metals | 1L | 500X (100 ml) | |
| MgSO4 7H20 | 200 mg | 10 g | Trace metal stock solution (500X) can be prepared at low pH (~3.0). Prepare a separate FeSO4 stock solution to prevent precipitation over time. Use 2ml of 500X stock to make 1L SASM. |
| MnSO4 7H20 | 10 mg | 0.5 g | |
| CuSO4 5H20 | 1 mg | 50 mg | |
| ZnSO4 4H20 | 1 mg | 50 mg | |
| FeSO4 5H20 | 10 mg | 0.5 g | |
| Amino Acids | |||
| All L-amino acids | 100 mg | TYR has low solubility so no stock solution can be prepared. Dissolve 100 mg of TYR first. Prepare 10 g/l stock solution containing amino acids ARG, THR, SER, HIS, MET, and ASN. Prepare 5 g/l stock solution containing amino acids PRO, ILE, LEU, VAL, and PHE. Prepare 5 g/l stock solution containing amino acids ASP, CYS, and TRP. Add ALA, GLY, LYS, GLU, and GLN, separately depending on the isotope labeling scheme. |