Select Publications

2025

Greathouse KL*, Choudhury A. Precision nutrition and the gut microbiome: Harnessing AI to revolutionize cancer prevention and therapy. Cell Host Microbe. 2025 Jun 11;33(6):766-776. doi: 10.1016/j.chom.2025.05.011. PMID: 40505617

Benjamin A. Weinberg, MD; Caitlin C. Murphy, PhD, MPH; David R. Freyer, DO, MS; K. Leigh Greathouse, PhD, MPH, MS, RD; Jan K. Blancato, PhD; Elena M. Stoffel, MD, MPH; Julia L.Drewes, PhD; Anne Blaes, MD, MS; John M. Salsman, PhD; Y. Nancy You, MD, MHSc; Hannah Arem, PhD; Reetu Mukherji, MD; Priyanka Kanth, MD, MS; Xin Hu, PhD; Anne Fabrizio, MD; Marion L. Hartley, PhD; Marios Giannakis, MD, PhD; John L. Marshall, MD. Smith, K.S.; Gudenkauf, L.M.; Hoogland, A.I.; Li, X.; Hoobler, R.; Playdon, M.C.; Gigic, B.; Rethinking the Rise of Early Onset Gastrointestinal Cancers: A Call to Action. JNCI Cancer Spectrum 2025. https://doi.org/10.1093/jncics/pkaf002 (accepted for publication)

2024

Chowdhury A, Scano C, Barton A, Kearney C, Greathouse KL. Targeted therapy of oncomicrobe F. nucleatum with bioengineered probiotic expressing guided antimicrobial peptide (gAMP). bioRxiv. 2024. doi: 10.1101/2024.10.29.620919

Small, B.J.; Gonzalez, B.D.; Oswald, L.B.; Byrd, D.A., Greathouse, K.L.; et al. Associations Between Dietary Patterns and Quality of Life in a Longitudinal Cohort of Colorectal Cancer Survivors. Nutrients 2024, 16, 3860. https://doi.org/10.3390/nu16223860

Madhur Wyatt, Ankan Choudhury, Gabriella Von Dohlen, Jeffery Heileson, Jeffrey Forsse, Sumudu Rajakaruna, Manja Zec, Malak Tfaily, and K. Leigh Greathouse*. Randomized control trial of moderate dose vitamin D alters microbiota stability and metabolite networks in healthy adults. Microbiol Spectr 2024, 12. https://doi.org/10.1128/spectrum.00083-24

Smith KS, Gudenkauf LM, Hoogland AI, Li X, Hoobler R, Playdon MC, Gigic B, Small BJ, Gonzalez BD, Oswald LB, Byrd DA, Greathouse KL, Ulrich CM, Li CI, Shibata D, Toriola AT, Peoples AR, Siegel EM, Figueiredo JC, Jim HSL, Crowder SL. Associations Between Dietary Patterns and Quality of Life in a Longitudinal Cohort of Colorectal Cancer Survivors. Nutrients. 2024. doi: 10.3390/nu16223860.

Stone JK, von Muhlinen N, Zhang C, Robles AI, Flis AL, Vega-Valle E, Miyanaga A, Matsumoto M, Greathouse KL, Cooks T, Trinchieri G, Harris CC. Acidovorax temperans skews neutrophil maturation and polarizes Th17 cells to promote lung adenocarcinoma development. Oncogenesis. 2024 doi: 10.1038/s41389-024-00513-6.

2023

Choudhury A, Ortiz P, Young M, Mahmud M, Stoffel R, Greathouse KL, Kearney C. Control of Helicobacter pylori with engineered probiotics secreting selective guided antimicrobial peptides. Microbiol Spectr. 2023. doi: 10.1128/spectrum.02014-23

Byrd D, Fan W, Greathouse KL, Wu M, Xie H, Wang X. The intratumor microbiome is associated with microsatellite instability. J Natl Cancer Inst. 2023; 115(8):989-993. doi: 10.1093/jnci/djad083

Sheikh A, Zechmann B, Sayes M, Taube J, Greathouse KL. A Preparation of bacterial outer membrane with osmium tetroxide and uranyl acetate co-stain enables improved structural determination by transmission electron microscopy. Microscopy. 2023. doi: 10.1093/jmicro/dfad027

2022

Greathouse KL, Wyatt M, Johnson AJ, et al. Diet-microbiome interactions in cancer treatment: Opportunities and challenges for precision nutrition in cancer. Neoplasia. 2022;29:100800. doi: 10.1016/j.neo.2022.100800

Crowder SL, Playdon MC, Gudenkauf LM, Ose J, Gigic B, Greathouse L, Peoples AR, Sleight AG, Jim HSL, Figueiredo JC. A Molecular Approach to Understanding the Role of Diet in Cancer-Related Fatigue: Challenges and Future Opportunities. Nutrients. 2022; 14(7):1496. doi: 10.3390/nu14071496

Fletcher E, Wiggs M, Greathouse KL, Morgan G, Gordon PM. Impaired proteostasis in obese skeletal muscle relates to altered immunoproteasome activity. Appl Physiol Nutr Metab. 2022;47(5):555-564. doi: 10.1139/apnm-2021-0764

Greathouse KL, Padgett RN, Petrosino J, Hastings-Tolsma M, Faucher MA. Exploration of Diet Quality by Obesity Severity in Association with Gestational Weight Gain and Distal Gut Microbiota in Pregnant African American Women: Opportunities for Intervention. Matern Child Health J. 2022 Apr;26(4):882-894. doi: 10.1007/s10995-021-03198-0.

2021

Greathouse KL, Choudhury A. Spiking immunotherapy with a bacterial cocktail brings T cells back to the fight. Cell Rep Med. 2021 Oct 19;2(10):100430. doi: 10.1016/j.xcrm.2021.100430

Aadil Sheikh, Joseph Taube, K Leigh Greathouse, Contribution of the Microbiota and their Secretory Products to Inflammation and Colorectal Cancer Pathogenesis: The Role of Toll-like Receptors, Carcinogenesis, 2021;, bgab060, doi: 10.1093/carcin/bgab060

Greathouse KL, Johnson AJ. Does caloric restriction prime the microbiome for pathogenic bacteria?. Cell Host Microbe. 2021;29(8):1209-1211. doi: 10.1016/j.chom.2021.07.009

Ismaeel A, Greathouse KL, Newton N, Miserlis D, Papoutsi E, Smith RS, Eidson JL, Dawson DL, Milner CW, Widmer RJ, Bohannon WT, Koutakis P. Phytochemicals as Therapeutic Interventions in Peripheral Artery Disease. Nutrients. 2021; 13(7):2143. doi: 10.3390/nu13072143

Wyatt M, Greathouse KL. Targeting Dietary and Microbial Tryptophan-Indole Metabolism as Therapeutic Approaches to Colon Cancer. Nutrients. 2021; 13(4):1189. doi: 10.3390/nu13041189

2020

Faucher MA, Greathouse KL, Hastings-Tolsma M, Padgett RN, Sakovich K, Choudhury A, Sheikh A, Ajami NJ, Petrosino

JF. Exploration of the Vaginal and Gut Microbiome in African American Women by Body Mass Index, Class of Obesity, and Gestational Weight Gain: A Pilot Study. American Journal of Perinatology 2019; 37(11): 1160-1172 doi: 10.1055/s-0039-1692715

Greathouse, K. L., Stone, J. K. & Harris, C. C. Cancer-Type-Specific Bacteria: Freeloaders or Partners? Cancer Cell 38, 158–160 (2020). doi: 10.1016/j.ccell.2020.06.017

Morales‐Marroquin, E, Hanson, B, Greathouse, L, de la Cruz‐Munoz, N, Messiah, SE. Comparison of methodological approaches to human gut microbiota changes in response to metabolic and bariatric surgery: A systematic review. Obesity Reviews. 2020; 21:e13025. doi: 10.1111/obr.13025

2019

Greathouse, K.L., Sinha, R. & Vogtmann, E. DNA extraction for human microbiome studies: the issue of standardization. Genome Biol 20, 212 (2019). doi: 10.1186/s13059-019-1843-8

Greathouse KL, White JR, Padgett RN, Perrotta BG, Jenkins GD, Chia N, Chen J. Gut microbiome meta-analysis reveals dysbiosis is independent of body mass index in predicting risk of obesity-associated CRC. BMJ Open Gastroenterology 2019;6:e000247. doi: 10.1136/bmjgast-2018-000247

2018

Morales E, Chen J, Greathouse KL. Compositional Analysis of the Human Microbiome in Cancer Research. Methods Mol Biol. 2019;1928:299-335. doi: 10.1007/978-1-4939-9027-6_16.

Greathouse, K.L., White, J.R., Vargas, A.J., Bliskovsky, V.V., Beck, J.A., von Muhlinen, N., Polley, E.C., Bowman, E.D., Khan, M.A., Robles, A.I., Cooks, T., Ryan, B.M., Padgett, N., Dzutsev, A.H., Trinchieri, G., Pineda, M.A., Bilke, S., Meltzer, P.S., Hokenstad, A.N., Stickrod, T.M., Walther-Antonio, M.R., Earl, J.P., Mell, J.C., Krol, J.E., Balashov, S.V., Bhat, A.S., Ehrlich, G.D., Valm, A., Deming, C., Conlan, S., Oh, J., Segre, J.A. & Harris, C.C. Interaction between the microbiome and TP53 in human lung cancer. Genome Biol 19, 123 (2018). doi: 10.1186/s13059-018-1501-6

2017

Daquigan, N., Seekatz, A.M., Greathouse, K.L., Young, V.B., White, J.R. High-resolution profiling of the gut microbiome reveals the extent of Clostridium difficile burden. npj Biofilms Microbiomes 3, 35 (2017). doi: 10.1038/s41522-017-0043-0

Greathouse KL, Faucher MA, Hastings-Tolsma M. The Gut Microbiome, Obesity, and Weight Control in Women’s Reproductive Health. Western Journal of Nursing Research. 2017;39(8):1094-1119. doi: 10.1177/0193945917697223

2015

Falana K, Knight R, Martin CR, Goldszmid R, Greathouse KL, Gere J, Young H, Kuo WP. Short Course in the Microbiome. J Circ Biomark. 2015;4:8. doi: 10.5772/61257.

Greathouse, K. L., Harris, C. C., & *Bultman, S. J. (2015). Dysfunctional families: Clostridium scindens and secondary bile acids inhibit the growth of Clostridium difficile. Cell Metab, 21(1), 9-10. doi: 10.1016/j.cmet.2014.12.016

2014

Robles AI, Yang P, Jen J, McClary AC, Calhoun K, Bowman ED, Vähäkangas K, Greathouse KL, Wang Y, Olivo-Marston S, Wenzlaff AS, Deng B, Schwartz AG, *Ryan BM. (2014) A DRD1 polymorphism predisposes to lung cancer among those exposed to secondhand smoke during childhood. Cancer Prev Res. 2014 Dec;7(12):1210-8. doi: 10.1158/1940-6207.CAPR-14-0158.

Greathouse, K., Chriqui, J., Moser, R., Agurs-Collins, T., & Perna, F. (2014). The association of soda sales tax and school nutrition laws: A concordance of policies. Public Health Nutrition, 17(10), 2201-2206. doi:10.1017/S1368980013003029

2012

Greathouse, K. L., Bredfeldt, T., Everitt, J. I., Lin, K., Berry, T., Kannan, K., Mittelstadt, M., Ho, S., & *Walker, C. L. (2012). Environmental estrogens differentially engage the histone methyltransferase EZH2 to increase risk of uterine tumorigenesis. Mol Cancer Res, 10(4), 546-557. doi: 10.1158/1541-7786.MCR-11-0605

2010

Bredfeldt, T. G., Greathouse, K. L., Safe, S. H., Hung, M. C., Bedford, M. T., & *Walker, C. L. (2010). Xenoestrogen-induced regulation of EZH2 and histone methylation via estrogen receptor signaling to PI3K/AKT. Mol Endocrinol, 24(5), 993-1006. doi: 10.1210/me.2009-0438

2008

Greathouse, K. L., Cook, J. D., Lin, K., Davis, B. J., Berry, T. D., Bredfeldt, T. G., & *Walker, C. L. (2008). Identification of uterine leiomyoma genes developmentally reprogrammed by neonatal exposure to diethylstilbestrol. Reprod Sci, 15(8), 765-778. doi: 10.1177/1933719108322440

2005

Greathouse, K. L., Samuels, M., DiMarco, N. M., & *Criswell, D. S. (2005). Effects of increased dietary fat and exercise on skeletal muscle lipid peroxidation and antioxidant capacity in male rats. Eur J Nutr, 44(7), 429-435. doi: 10.1007/s00394-005-0548-9

MANUSCRIPTS UNDER REVIEW FOR PUBLICATION

Leticia Cherpe de Souza, K. Leigh Greathouse, LesLee Funderburk (under review). The Impact of Dietary Acculturation and Food Availability in the Body Composition of Division I Female International Student-Athletes. Journal of American College Health